Development of a Molecular Marker for Anti-ALS Inhibitor-like Herbicides and Its Applications

doi:10.3969/j.issn.1006-8082.2024.04.004

China Rice ›› 2024, Vol. 30 ›› Issue (4): 17-23.DOI: 10.3969/j.issn.1006-8082.2024.04.004

• Special Thesis & Basic Research • Previous Articles Next Articles

Development of a Molecular Marker for Anti-ALS Inhibitor-like Herbicides and Its Applications

SANG Shifei¹^,²^,^#, SUN Xiaohan¹^,²^,^#, YAO Guoqin¹^,², MA Tengyun¹^,², ZHANG Yijing¹^,², ZHENG Yangyang³, FENG Liuchun¹^,², JI Shengdong¹^,²^,^*()

¹College of Life Sciences, Henan Normal University, Xinxiang, Henan 453007, China
²Henan Crop Genetic Improvement and Germplasm Innovation Engineering Research Center, Xinxiang, Henan 453007, China
³Xinxiang Huanong Seed Industry Co., Ltd, Xinxiang, Henan 453007, China

Received:2023-08-31 Online:2024-07-20 Published:2024-07-16
Contact: JI Shengdong
About author:First author contact:
^#Co-first auhtor;

抗ALS抑制剂类除草剂分子标记的开发及应用

桑世飞¹^,²^,^#, 孙晓涵¹^,²^,^#, 姚国琴¹^,², 马腾云¹^,², 章怡静¹^,², 郑阳阳³, 丰柳春¹^,², 姬生栋¹^,²^,^*()

¹河南师范大学生命科学学院，河南新乡 453007
²河南省作物遗传改良与种质创新工程研究中心，河南新乡453007
³新乡市华农种业有限公司，河南新乡 453007

通讯作者: 姬生栋
作者简介:第一联系人：
^#共同第一作者；
基金资助:
国家自然科学基金青年基金项目(32201823);新乡市重大科技专项(22ZD001);河南省科技攻关计划项目(212102110064)

Abstract

Abstract:

Weeds seriously restrict rice production, and cultivating new varieties of rice with broad-spectrum herbicide resistance and acetyllactate synthase (ALS) inhibitors is one of an effective ways to deal with the harm of weeds in rice fields. The difference sites between the anti-ALS inhibitor herbicide Jinjing 818 and the sensitive herbicide Nipponbare ALS gene were identified, and specific molecular markers were developed. Herbicide spraying and molecular marker-assisted selection (MAS) were carried out on the hybrid offspring of Jinjing 818 and Jindao 372 with ALS-resistant genotypes as parents. Two pairs of molecular markers, 818ALSF/R and HDALSF/R were developed, which could quickly distinguish three genotypes of homozygous dominant, heterozygous and homozygous genotypes, and were consistent with the herbicide spraying results. Two excellent rice germplasm resource materials, 22S9 and 22S4, with excellent herbicide resistance, were bred. Germplasm resource materials with ALS herbicide resistance gene can be quickly screened out by MAS of hybrid offspring, and the screening efficiency in rice germplasm of anti-ALS inhibitor herbicides can be improved.

Key words: ALS herbicide, acetolactate synthase, rice, herbicide resistance

摘要：

杂草严重制约水稻生产，培育具有乙酰乳酸合成酶（acetolactate synthase，ALS）抑制剂的广谱抗除草剂水稻新品种是应对稻田杂草危害的有效途径之一。本研究鉴定了抗ALS抑制剂类除草剂金粳818与感除草剂日本晴ALS基因的差异位点，开发特异分子标记。对以携带ALS抗性基因型的金粳818、津稻372为亲本的杂交后代进行除草剂喷施和分子标记辅助选择。开发了818ALSF/R、HDALSF/R两对分子标记，可以快速区分纯合显性、杂合、纯合隐性3种基因型，且与除草剂喷施结果一致；选育得到22S9和22S4两份抗除草剂优异水稻种质资源材料。通过对杂交后代分子标记辅助选择可快速筛选出具有ALS除草剂抗性基因的种质资源材料，提高了在抗ALS抑制剂类除草剂水稻种质中的筛选效率。

关键词: ALS除草剂, 乙酰乳酸合成酶, 水稻, 除草剂抗性

CLC Number:

S511.053

SANG Shifei, SUN Xiaohan, YAO Guoqin, MA Tengyun, ZHANG Yijing, ZHENG Yangyang, FENG Liuchun, JI Shengdong. Development of a Molecular Marker for Anti-ALS Inhibitor-like Herbicides and Its Applications[J]. China Rice, 2024, 30(4): 17-23.

桑世飞, 孙晓涵, 姚国琴, 马腾云, 章怡静, 郑阳阳, 丰柳春, 姬生栋. 抗ALS抑制剂类除草剂分子标记的开发及应用[J]. 中国稻米, 2024, 30(4): 17-23.

Figures/Tables 9

References 26

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Development of a Molecular Marker for Anti-ALS Inhibitor-like Herbicides and Its Applications

抗ALS抑制剂类除草剂分子标记的开发及应用

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