China Rice ›› 2021, Vol. 27 ›› Issue (4): 92-100.DOI: 10.3969/j.issn.1006-8082.2021.04.019

• Special Thesis & Basic Research • Previous Articles     Next Articles

Development and Application of Genome Editing Technology in Rice

Jun REN#(), Yuexuan CAO#, Yong HUANG, Huirong DONG, Qing LIU, Kejian WANG()   

  1. China National Rice Research Institute/State Key Laboratory of Rice Biology, Hangzhou 310006,China
  • Received:2021-05-13 Online:2021-07-20 Published:2021-07-20
  • About author:

    #Co-first author: 82101172206@caas.cn

基因编辑技术及其水稻中的发展和应用

任俊#(), 曹跃炫#, 黄勇, 董慧荣, 刘庆, 王克剑*()   

  1. 中国水稻研究所/水稻生物学国家重点实验室,杭州 310006
  • 通讯作者: 王克剑
  • 作者简介:

    #共同第一作者:82101172206@caas.cn

  • 基金资助:
    国家自然科学基金(31901523)

Abstract:

As an emerging genome editing system, the CRISPR/Cas system has been widely used in plant functional genome research and variety improvement due to its simplicity, efficiency, and high specificity. In this article, we systematically summarized the development and application of genome editing tools in plants. We focused on the latest advances in plant germplasm and variety improvement based on genome editing technology, and described a new strategy for plant breeding based on genome editing technology, which is difficult to achieve in traditional breeding. Finally, we also discussed the challenges that genome editing technology will face in future crop and food production.

Key words: rice, CRISPR/Cas, quality, biological resistance, abiotic resistance, haploid breeding, apomixes, directed evolution

摘要:

CRISPR/Cas系统作为一种新兴的基因编辑系统,以其简单、高效、特异性高等特点已广泛应用于植物功能基因组研究和品种改良。在本文中,首先,我们系统总结了CRISPR/Cas技术及其衍生技术在植物中的开发和优化;其次,重点介绍了基于基因组编辑技术的水稻种质和品种改良的最新进展,并描述了基于基因编辑技术的水稻育种新策略,这是传统育种很难实现的;最后,还讨论了基因编辑技术在未来作物和粮食生产中所要面对的挑战。

关键词: 水稻, CRISPR/Cas, 稻米品质, 生物抗性, 非生物抗性, 单倍体育种, 无融合生殖, 定向进化

CLC Number: