中国稻米

中国稻米 ›› 2024, Vol. 30 ›› Issue (6): 105-109.DOI: 10.3969/j.issn.1006-8082.2024.06.018

• 品种与技术 • 上一篇    下一篇

聚合抗稻瘟病基因Pigm和抗白叶枯病基因Xa23改良粳稻宁84抗性

黄宣1(), 邱海萍2,*(), 严成其1, 姜洁锋1, 施贤波1, 叶朝辉1   

  1. 1宁波市农业科学研究院，浙江 宁波 315040
    2浙江省农业科学院，杭州 310021
  • 收稿日期:2024-01-12 出版日期:2024-11-20 发布日期:2024-11-19
  • 通讯作者: *qiuhping@163.com
  • 作者简介:252919163@qq.com
  • 基金资助:
    宁波市科技创新2025重大专项(2022Z175);宁波市科技创新2025重大专项(2021Z001);浙江省“十四五”农业新品种选育重大科技专项(2021C02063-5)

Improvement of the Resistance of Japonica Rice to Blast and Bacterial Blight by Pyramiding Resistance Gene Pigm and Xa23

HUANG Xuan1(), QIU Haiping2,*(), YAN Chengqi1, JIANG Jiefeng1, SHI Xianbo1, YE Chaohui1   

  1. 1Ningbo Academy of Agricultural Sciences, Ningbo, Zhejiang 315040, China
    2Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

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摘要：

稻瘟病和白叶枯病是水稻两大重要病害，严重影响水稻产量和品质。培育抗病品种是降低稻瘟病和白叶枯病危害最经济有效的措施。本研究结合分子标记辅助选择技术和GSR40K芯片检测，将广谱抗性基因Pigm和Xa23导入粳稻品种宁84中，获得3份携带双基因的株系。抗性鉴定结果表明，导入Pigm基因显著提高了宁84对苗瘟和穗颈瘟的抗性，3个株系在苗期对41个稻瘟病菌株的抗性频率为63.4%~97.6%。导入Xa23的宁84株系对白叶枯病均达到“抗”以上级别，抗性显著提高。对改良株系的农艺性状考察结果表明，部分株系粒宽、千粒重和穗长显著增加，其他农艺性状及产量与宁84差异不显著。GSR40K基因芯片分析结果显示，3个改良株系的背景回复率为94.89%~96.58%。本研究结果说明，导入Pigm和Xa23能显著提高宁84对稻瘟病和白叶枯病的抗性水平，且对农艺性状和产量没有明显的负面影响。

关键词: 粳稻, 稻瘟病, 白叶枯病, 基因聚合, 抗性改良

Abstract:

Rice blast and bacterial blight are two significant diseases in rice, causing huge yield and quality losses. Breeding resistant varieties is the most cost-effective approach to reduce their harm. In this study, using molecular marker-assisted selection and GSR40K chip detection, we introduced the rice broad-spectrum resistance genes Pigm and Xa23 into the japonica rice variety Ning84. We obtained three new lines carrying the two genes. The resistance identification results showed that Pigm introduction significantly increased the resistance to seedling blast and panicle blast of Ning 84, the resistance frequency of the three new lines to 41 rice blast strains during seedling stage ranged from 63.4% to 97.6%. The resistance to leaf blight of the Ning 84 improved lines imported into Xa23 all reached the level of “resistance” or above, its resistance significantly improved. The results of examining the agronomic traits of the improved lines showed that some lives had significantly increased grain width, thousand grain weight, and spike length, while other agronomic traits and yield did not differ significantly from Ning 84. The results of GSR40K SNP gene chip analysis showed that the background response rate of the three improved lines was 94.89%-96.58%. The results of this study indicated that the introduction of Pigm and Xa23 could significantly increase the resistance level of Ning 84 to rice blast and leaf blight, and has no obvious negative effect on agronomic traits and yield.

Key words: japonica rice, rice blast, bacterial blight, gene pyramiding, resistance improvement

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