一种抗稻曲病菌的摩氏假单胞菌JP2-207及其抗性机制初探

doi:10.3969/j.issn.1006-8082.2024.02.003

摘要/Abstract

摘要：

从水稻根际表面土壤分离到一株能有效抑制稻曲病菌（Ustilaginoidea virens）生长的细菌菌株JP2-207。16s rRNA基因片段扩增及系统发育树分析表明，JP2-207属于摩氏假单胞菌（Pseudomonas mosselii）。测序分析显示，JP2-207全基因组为1个大小5 702 443 bp的环状DNA，包含5 154个蛋白编码基因，22个rRNA和78个tRNA基因。生物信息学分析预测了9个与次生代谢产物生物合成相关的基因簇，其中多个基因簇产生的代谢物被报道对植物病原菌具有抑制活性。本研究为摩氏假单胞菌JP2-207及其代谢产物防治水稻稻曲病的研究奠定了初步的理论基础。

关键词: 摩氏假单胞菌, 水稻, 稻曲病, 次生代谢产物, 生物合成基因簇, 生物防治

Abstract:

Pseudomonas mosselii JP2-207 was isolated from the soil of the rhizosphere of rice plants, this strain showed obvious inhibitory activity against Ustilaginoidea virens, an important rice pathogen. The complete genome of P. mosselii JP2-207 consists of 5 702 443 bp with a circle chromosome, 5 154 protein-coding genes, 22 rRNA genes, and 78 tRNA. The genomic analysis revealed that 9 candidate gene clusters were involved in the biosynthesis of secondary metabolites. Together, this study provides preliminary results for exploring the antagonistic mechanism of rice false smut by P. mosselii JP2-207 and its secondary metabolites.

Key words: Pseudomonas mosselii, rice, rice false smut disease, metabolite, gene clusters, biocontrol

中图分类号:

刘思慧, 明珂, 陈国庆, 冯国忠. 一种抗稻曲病菌的摩氏假单胞菌JP2-207及其抗性机制初探[J]. 中国稻米, 2024, 30(2): 13-17.

LIU Sihui, MING Ke, CHEN Guoqing, FENG Guozhong. A Preliminary Study on the Mechanism of Pseudomonas mosselii JP2-207 Against Rice False Smut Fungus Ustilaginoidea virens[J]. China Rice, 2024, 30(2): 13-17.

图/表 6

图1 JP2-207对稻曲病菌的抑制活性左侧显示了JP2-207对菌丝体生长的抑制状况；右侧是生长抑制的直方图，柱状图代表三个重复的标准误差。根据t检验，以不同的英文字母显示CK和JP2-207处理之间的显著差异（P<0.05）。

图2 基于16S rRNA基因序列的摩氏假单胞菌JP2-207系统发育学分析使用neighbor-joining方法和kimura 2-参数模型计算进化距离，在分支节点上标注1000自展值（>50%）。该进化树是按比例绘制的，其分支长度与用于推断系统发育树的进化距离的单位相同。绿假单胞菌OK2的16S rRNA基因序列被用作外群。GenBank登录号显示在括号中。Bar，每个核苷酸位置0.002个核苷酸取代。本研究中的菌株使用粗体标注。

图3 JP2-207全基因组

表1 JP2-207全基因组信息

图4 COG功能注释 JP2-207共有4 686个COG功能分类，包含于24个类群中，包括调控细胞周期、氨基酸转运代谢、核酸转运代谢、脂类转运和代谢及糖类运输代谢等。

表2 JP2-207代谢产物合成基因簇预测

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