中国稻米

中国稻米 ›› 2024, Vol. 30 ›› Issue (1): 47-52.DOI: 10.3969/j.issn.1006-8082.2024.01.008

• 专论与研究 • 上一篇    下一篇

外源氨基酸对层出镰刀菌菌丝生长和伏马毒素合成的影响

梁文豪, 胡时开, 圣忠华, 魏祥进, 焦桂爱, 邵高能, 谢黎虹, 王玲*(), 唐绍清*(), 胡培松*()   

  1. 中国水稻研究所/水稻生物育种全国重点实验室，杭州 310006
  • 收稿日期:2023-06-20 出版日期:2024-01-20 发布日期:2024-01-23
  • 通讯作者: *wangling03@caas.cn；tangshaoqing@caas.cn；peisonghu@126.com
  • 基金资助:
    国家自然科学基金(32188102);国家自然科学基金(31800133);浙江省重点研发项目(2021C02063);浙江省重点研发项目(2021C02056);浙江省重点研发项目(2022C02011)

Effects of Exogenous Amino Acids on the Mycelial Growth and Fumonisins Production of Fusarium proliferatum

LIANG Wenhao, HU Shikai, SHENG Zhonghua, WEI Xiangjin, JIAO Gui’ai, SHAO Gaoneng, XIE Lihong, WANG Ling*(), TANG Shaoqing*(), HU Peisong*()   

  1. China National Rice Research Institute/ State Key Laboratory of Rice Biology and Breeding, Hangzhou 310006, China

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摘要：

由层出镰刀菌引起的水稻穗腐病，不仅造成水稻产量损失，而且因产生真菌毒素而导致稻米品质下降。本研究通过在生长培养基中添加外源氨基酸，评价其对层出镰刀菌菌丝生长和伏马毒素合成的影响。结果表明，不同氨基酸和不同添加量对菌丝生长均有影响。与未添加氨基酸的对照相比，在MM培养基上添加L-精氨酸、L-谷氨酸、L-脯氨酸和L-瓜氨酸，在PDA培养基上添加L-谷氨酸，均抑制菌丝生长。添加氨基酸显著提高了伏马毒素含量，且随着氨基酸浓度升高，在PDA中产毒量减少，在MM中则呈增加趋势。外源氨基酸明显改变了伏马毒素生物合成基因在转录水平上的表达，但基因表达模式与毒素含量并不一致。综合分析表明，外源氨基酸会影响层出镰刀菌菌丝生长和伏马毒素含量，且不同氨基酸组分与菌丝生长和伏马毒素合成的关联性不同。

关键词: 水稻, 穗腐病, 层出镰刀菌, 外源氨基酸, 菌丝生长, 伏马毒素

Abstract:

Rice spikelet rot disease caused by Fusarium proliferatum not only leads to yield reduction, but also contaminates rice grains with mycotoxins. In this study, the effects of exogenous amino acids on the mycelial growth and fumonisins biosynthesis of F. proliferatum were determined through the addition of amino acids to the growth media. The results showed that the growth of F. proliferatum was affected by different exogenous additives and different addition amounts compared to the mock control. The mycelial growth of F. proliferatum was obviously inhibited in MM media supplied with L-arginine, L-glutamate, L-proline and L-citrulline, respectively, and in PDA media supplied with L-glutamate. Fumonisins production was significantly increased by different kinds of amino acids. Fumonisins content tended to reduce in PDA media and increase in MM media with the higher concentration of amino acids. There were significant changes in expression levels of the fumonisins biosynthetic (fum) genes treated with exogenous amino acids, but expression patterns of fum genes were not consistent with fumonisins production. The results demonstrated that the mycelial growth and fumonisins production of F. proliferatum were influenced by the quantity and type of amino acids, and the correlation between different amino acid composition and mycelial growth fumonisins biosynthesis was different.

Key words: rice, spikelet rot disease, Fusarium proliferatum, exogenous amino acids, mycelial growth, fumonisins

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