中国稻米

中国稻米 ›› 2023, Vol. 29 ›› Issue (2): 98-102.DOI: 10.3969/j.issn.1006-8082.2023.02.020

• 品种与技术 • 上一篇    下一篇

应用二次通用旋转组合设计禾长蠕胞菌制剂配方

张建萍1(), 段桂芳2, 杨爽1, 余柳青1, 陆永良1, 唐伟1()   

  1. 1.中国水稻研究所/水稻生物育种全国重点实验室，杭州 310006
    2.上海晓明检测技术服务有限公司，上海 201612
  • 收稿日期:2022-09-22 出版日期:2023-03-20 发布日期:2023-03-14
  • 通讯作者: *tangwei@caas.cn
  • 作者简介:zhangjianping@caas.cn

Application of Uniform-Precision Rotatable Central Composite Design on Formulation of Helminthosporium gramineum Rabenh. f.sp. echinochloae

ZHANG Jianping1(), DUAN Guifang2, YANG Shuang1, YU Liuqing1, LU Yongliang1, TANG Wei1()   

  1. 1. China National Rice Research Institute/State Key Laboratory of Rice Biology and Breeding, Hangzhou 310006, China
    2. Shanghai Greentech Laboratory Co., Ltd. Shanghai 201612, China
  • Received:2022-09-22 Online:2023-03-20 Published:2023-03-14
  • Contact: *tangwei@caas.cn
  • About author:zhangjianping@caas.cn

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摘要：

禾长蠕孢菌（Helminthosporium gramineum Rabenh. f.sp.echinochloae）是一种稻田微生物除草潜力菌。为提高其在田间应用的效果，本文以稗草为靶标杂草，前期筛选到的助剂（增效剂、溶剂油、稳定剂、乳化剂）为编码因子，采用二次通用旋转组合设计配方，根据各配方对稗草的致死率建立回归方程，并通过方差分析，对方程进行优化，最终优化得到禾长蠕孢菌孢子制剂的配方为：1.0 g孢子粉中添加0.25 mL大豆油、0.49 g羧甲基纤维素钠、0.1 mL SP-20，增效剂10%吡嘧磺隆WP 6.56 mg和2.5%五氟磺草胺OD 26 μL。研究结果有助于HGE菌后续商品化应用。

关键词: 水稻, 微生物除草剂, 禾长蠕胞菌, 孢子, 稗草, 制剂

Abstract:

Helminthosporium gramineum Rabenh. f. sp. echinochloae (HGE), evaluated as a potential pathogenic fungus strain, could be applied to control barnyard grass in paddy field. For improving the weed control efficiency of HGE in paddy field, the barnyard grass was used as target weed and the adjuvants screened previously was designated as experimental factors. The uniform-precision rotatable central composite design was adopted to establish the regression equation of the lethal rate of barnyard grass in different formulations. The optimal formulation of HGE spore preparation containing 1.0 g spore powder, 0.25 mL soybean oil, 0.49 g carboxy methyl cellulose sodium (CMC), 0.10 mL SP-20 and synergists which dosage was 6.56 mg 10% Pyrazosulfuron-ethyl WP and 26 μL 2.5% Penoxsulam OD was achieved through the analysis of variance on the test results and the optimization of the mathematical model. These results provided useful formulation for next commercialized application of HGE.

Key words: rice, bioherbicide, Helminthosporium gramineum Rabenh. f. sp. echinochloae, spore, barnyard grass, formulation

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