关键词: 木霉, 代谢物质, 抑制作用, 挥发性物质, 几丁质酶

Abstract: The antagonistic activities of Trichoderma harzianum and Trichoderma brevis against four pathogens causing rice seedling blight in cold regions were analyzed by confrontation culture, fermentation broth, non-volatile and volatile inhibitors. The results showed that both Trichoderma harzianum and Trichoderma brevis inhibited four pathogens to some extent. In confrontation culture, Trichoderma harzianum inhibited Fusarium oxysporum with the highest inhibition rate of 79.04% and Trichoderma brevis inhibited Fusarium graminearum with the highest inhibition rate of 88.65%. The non-volatile substances of Trichoderma spp. had strong inhibitory effects on four pathogenic bacteria, among which Fusarium graminearum had the strongest inhibitory effects, with inhibition rates of 66.78% and 70.12%, respectively. The chitinase activity of Trichoderma harzianum was higher than that of Trichoderma brevis. The results will lay a foundation for the research and development of Trichoderma complex fungus preparation to control rice seedling blight.

Key words: Trichoderma, metabolic substances, inhibitory effects, volatile substances, chitinase